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产品名称:

RAMOS (RA 1)细胞,人B淋巴瘤细胞

产品型号: 产品时间: 2018-12-03
RAMOS (RA 1)细胞,人B淋巴瘤细胞
细胞是生物体结构和功能的基本单位,细胞的特殊性决定了个体的特殊性,因此,对细胞的深入研究是揭开生命奥秘、改造生命和征服疾病的关键。细胞生物学已经成为当代生物科学中发展较快的一门*学科,是生物、农学、医学、畜牧、水产和许多生物相关专业的一门必修课程。50年代以来诺贝尔生理与医学奖大都授予了从事细胞生物学研究的科学家。

产品概述

RAMOS (RA 1)细胞,人B淋巴瘤细胞

比特棋牌CHANPINMINGCHENG:RAMOS (RA 1)

XIBAOXINGTAI:LINBAMUXIBAOYANG;YUANXING

ZUZHILAIYUAN:LINBAXIBAO

比特棋牌CHUANDAIQINGKUANG:P3-P5  

细胞数量:1~3*106比特棋牌细胞/25cm2培养瓶

比特棋牌PEIYANGTIAOJIAN:RPMI1640(w/oHepes)10%FBS

CHUANDAIFANGFA:1:2~1:4CHUAN,2-3TIAN1CI

SHENGZHANGTIAOJIAN:95%KONGQI+5%ERYANGHUATAN 37SHESHIDU

SHENGZHANGZHUANGTAI:XUANFUSHENGZHANG

CUNCHUTIAOJIAN:90%FBS+10%DMSO

RAMOS (RA 1)细胞,人B淋巴瘤细胞

比特棋牌DUIYUTIEBIXIBAO,RUOXIBAOPIAOFU:PEIYANGPINGBUKAIFENG,YONG75%JIUJINGCASHIHOUPINGTANGZHIYUPEIYANGXIANGNEI。CIRIGUANCHA,RUXIBAODABUFENYOUTIEHUIPINGDI,BIAOMINGXIBAOHUOLIZHENGCHANG,SHENGYUPIAOFUDEXIBAOKEYIQUDIAO,HUANCHENGXINXIANDEPEIYANGJI;RUXIBAOHAIBUTIEBI,JIANGXIBAOLIXINSHOUJIYIDAOXINDEPEIYANGPINGZHONG,YUANPEIYANGPINGJIABUFENXINXIANPEIYANGYEJIXUPEIYANG,ZHONGJIANZHUYIGUANCHA,WOMENDEJISHURENYUANHUIYIZHIGENZONGZHIDAO,ZHIDAOWENTIJIEJUE。DUIYUXUANFUXIBAO:SHOUDAOXIBAOHOU,JIANGXIBAOQUANBULIXIN,QUDIAOJIUDEPEIYANGJI,HUANCHENGXINXIANDEPEIYANGJIJIXUPEIYANG。

特别注意:(如使用公共实验室或者初次接触,建议添加双抗培养)

(1)TIEBIXIBAOSHOUDAODANGTIANYOUSHAOLIANGHUOMEIYOUPIAOHUDEXIBAOKEYIDANGTIANHUANYE,YINWEILUTUDIANBOZAOCHENGDALIANGPIAOHUDEQINGKUANGSHI,QINGJIANGXIBAOHUANYEHOUFANGZHIPEIYANGXIANGFUYUDAODIERTIANZAIZUOXIAOHUACHUANDAI,QINGYOUXIANXUANZEZHIJING6cmDEPEIYANGMINJINXINGCHUANDAIPEIYANG

(2)SHOUDAOXIBAOHOUQINGJINKUAIGENGHUANWEIHAN10%XUEQINGDEXINXIANPEIYANGJI,RUYINTESHUQINGKUANGXUYAOJIXUSHIYONGYUANPING,QINGZAIYUANPINGPEIYANGJIZHONGEWAITIANJIA10%DEXUEQING,(YUANPINGPEIYANGJIDEJIXUSHIYONGSHIJIANZUIZHANGBUYICHAOGUO72XIAOSHI)

(3)RUQIANSHOUSHICHUXIANPEIYANGPINGBIPOLIE,LOUYEDENGQINGKUANGQINGJISHIZUOHAOZHAOPIANJILUBINGLIANXISUERJISHURENYUAN

比特棋牌XIBAOJIESHOUHOUDECAOZUOLIUCHENGYUZHUYISHIXIANG:

1)TIEBIXIBAOSHENGZHANGHUANMAN;SHIDANGTIGAOXUEQINGNONGDU(ZUIGAOBUNENGCHAOGUO20%),HUOKEGENJUGAIXIBAOSHENGZHANGTEXINGSHENGZHANGMIDU,KAOLVYIMEIXIAOHUAHOU,ZHUANYIDAOXINDEPEIYANGPINGJIXUPEIYANG

比特棋牌2)RUGUOXIBAOWEITIEBIXIBAO,ERSHOUDAOSHICHENGXUANFUHUOZHEBUFENXUANFUDEZHUANGTAI,QINGJIANGXUANFUDEXIBAOJISHILIXIN,JIA15%XUEQINGDEWANQUANPEIYANGJIDAOXINDEPEIYANGMIN/PINGJIXUPEIYANG3TIAN;TONGSHIYUANPEIYANGPINGZHONGSHENGXIADETIEBIXIBAOGENGHUANWEI15%XUEQINGDEWANQUANPEIYANGJIPEIYANG3TIAN。3TIANHOURUOYUANPINGHUOZHEXINPINGZHONGDEXIBAODOUMEIYOUCHUXIANZENGZHIERSHIJIXUTUOLUOSIWANG,QINGJISHILIANXISHIYANSHIJISHURENYUANHUIGENJINJIEJUE

3)SHENGZHANGBUJUN:TIEBIXIBAORUOCHUXIANFENBUBUJUN,CHENGDAOZHUANGSHENGZHANG,KEJIANGXIBAOJINXINGXIAOHUA,ZHONGXUANDASANXIBAO,JIARUXINXIANPEIYANGJIJINXINGPEIYANG

比特棋牌XIBAOCHANGGUIPEIYANGCHUANDAILIUCHENG(QINGYANGEZUNZHAOWUJUNCAOZUO)

比特棋牌(1)XICHUYUANPEIYANGPINGZHONGDEPEIYANGJI,PBSHUANCHONGYERUNXIXIBAOLIANGCI,JIA2-3ml 0.25%YIMEIJINXINGXIAOHUAXIBAO(ZHUYIBAWOXIAOHUASHIJIAN,TONGCHANGKONGZHIZAI1-2min)

(2)ZHUYIPEIYANGJIPHZHIBIANHUAQINGKUANG,DINGQIHUANYE(MEIZHOU2-3CI),DAIXIBAOMIDUDADAO80%YIHOUZHONGFU1XIANGCAOZUOHUOZHEDONGCUN

(3)QUBUFENXIBAOXUANYEZHUANYIDAOXINDEPEIYANGMIN/PINGZHONG,TIANJIASHIDANGDEWANQUANPEIYANGJI,BAXIBAOXUANYEDAYUN,YUPEIYANGXIANGZHONGPEIYANG

(4)JINGXIAGUANCHAXIAOHUAQINGKUANG,ZAIHS505.TRENLINBAJIECHENGXIANWEIYANGXIBAOSHENGZHANGTEXINGBIANYUANSUOXIAO,TIEBISONGDONGSHI(BUJIANYIXIAOHUADAOXIBAOPIAOFU)QUDIAOYIMEI,JIA6-8mlWANQUANPEIYANGJI,QINGQINGCHUIDAXIBAOCENG,JINLIANGBAXIBAOCENGCHUILUO,CHUISAN。

比特棋牌HUO   HAO:SUER0009(XR)

比特棋牌CHANPINMINGCHENG:RAMOS (RA 1)

比特棋牌XIBAOXINGTAI:LINBAMUXIBAOYANG;YUANXING

ZUZHILAIYUAN:LINBAXIBAO

比特棋牌CHUANDAIQINGKUANG:P3-P5  

细胞数量:1~3*106细胞/25cm2培养瓶

 

PEIYANGTIAOJIAN:RPMI1640(w/oHepes)10%FBS

CHUANDAIFANGFA:1:2~1:4CHUAN,2-3TIAN1CI

SHENGZHANGTIAOJIAN:95%KONGQI+5%ERYANGHUATAN 37SHESHIDU

SHENGZHANGZHUANGTAI:XUANFUSHENGZHANG

CUNCHUTIAOJIAN:90%FBS+10%DMSO

 

DUIYUTIEBIXIBAO,RUOXIBAOPIAOFU:PEIYANGPINGBUKAIFENG,YONG75%JIUJINGCASHIHOUPINGTANGZHIYUPEIYANGXIANGNEI。CIRIGUANCHA,RUXIBAODABUFENYOUTIEHUIPINGDI,BIAOMINGXIBAOHUOLIZHENGCHANG,SHENGYUPIAOFUDEXIBAOKEYIQUDIAO,HUANCHENGXINXIANDEPEIYANGJI;RUXIBAOHAIBUTIEBI,JIANGXIBAOLIXINSHOUJIYIDAOXINDEPEIYANGPINGZHONG,YUANPEIYANGPINGJIABUFENXINXIANPEIYANGYEJIXUPEIYANG,ZHONGJIANZHUYIGUANCHA,WOMENDEJISHURENYUANHUIYIZHIGENZONGZHIDAO,ZHIDAOWENTIJIEJUE。DUIYUXUANFUXIBAO:SHOUDAOXIBAOHOU,JIANGXIBAOQUANBULIXIN,QUDIAOJIUDEPEIYANGJI,HUANCHENGXINXIANDEPEIYANGJIJIXUPEIYANG。

特别注意:(如使用公共实验室或者初次接触,建议添加双抗培养)

(1)TIEBIXIBAOSHOUDAODANGTIANYOUSHAOLIANGHUOMEIYOUPIAOHUDEXIBAOKEYIDANGTIANHUANYE,YINWEILUTUDIANBOZAOCHENGDALIANGPIAOHUDEQINGKUANGSHI,QINGJIANGXIBAOHUANYEHOUFANGZHIPEIYANGXIANGFUYUDAODIERTIANZAIZUOXIAOHUACHUANDAI,QINGYOUXIANXUANZEZHIJING6cmDEPEIYANGMINJINXINGCHUANDAIPEIYANG

(2)SHOUDAOXIBAOHOUQINGJINKUAIGENGHUANWEIHAN10%XUEQINGDEXINXIANPEIYANGJI,RUYINTESHUQINGKUANGXUYAOJIXUSHIYONGYUANPING,QINGZAIYUANPINGPEIYANGJIZHONGEWAITIANJIA10%DEXUEQING,(YUANPINGPEIYANGJIDEJIXUSHIYONGSHIJIANZUIZHANGBUYICHAOGUO72XIAOSHI)

(3)RUQIANSHOUSHICHUXIANPEIYANGPINGBIPOLIE,LOUYEDENGQINGKUANGQINGJISHIZUOHAOZHAOPIANJILUBINGLIANXISUERJISHURENYUAN

XIBAOJIESHOUHOUDECAOZUOLIUCHENGYUZHUYISHIXIANG:

1)TIEBIXIBAOSHENGZHANGHUANMAN;SHIDANGTIGAOXUEQINGNONGDU(ZUIGAOBUNENGCHAOGUO20%),HUOKEGENJUGAIXIBAOSHENGZHANGTEXINGSHENGZHANGMIDU,KAOLVYIMEIXIAOHUAHOU,ZHUANYIDAOXINDEPEIYANGPINGJIXUPEIYANG

2)RUGUOXIBAOWEITIEBIXIBAO,ERSHOUDAOSHICHENGXUANFUHUOZHEBUFENXUANFUDEZHUANGTAI,QINGJIANGXUANFUDEXIBAOJISHILIXIN,JIA15%XUEQINGDEWANQUANPEIYANGJIDAOXINDEPEIYANGMIN/PINGJIXUPEIYANG3TIAN;TONGSHIYUANPEIYANGPINGZHONGSHENGXIADETIEBIXIBAOGENGHUANWEI15%XUEQINGDEWANQUANPEIYANGJIPEIYANG3TIAN。3TIANHOURUOYUANPINGHUOZHEXINPINGZHONGDEXIBAODOUMEIYOUCHUXIANZENGZHIERSHIJIXUTUOLUOSIWANG,QINGJISHILIANXISHIYANSHIJISHURENYUANHUIGENJINJIEJUE

比特棋牌3)SHENGZHANGBUJUN:TIEBIXIBAORUOCHUXIANFENBUBUJUN,CHENGDAOZHUANGSHENGZHANG,KEJIANGXIBAOJINXINGXIAOHUA,ZHONGXUANDASANXIBAO,JIARUXINXIANPEIYANGJIJINXINGPEIYANG

XIBAOCHANGGUIPEIYANGCHUANDAILIUCHENG(QINGYANGEZUNZHAOWUJUNCAOZUO)

(1)XICHUYUANPEIYANGPINGZHONGDEPEIYANGJI,PBSHUANCHONGYERUNXIXIBAOLIANGCI,JIA2-3ml 0.25%YIMEIJINXINGXIAOHUAXIBAO(ZHUYIBAWOXIAOHUASHIJIAN,TONGCHANGKONGZHIZAI1-2min)

比特棋牌(2)ZHUYIPEIYANGJIPHZHIBIANHUAQINGKUANG,DINGQIHUANYE(MEIZHOU2-3CI),DAIXIBAOMIDUDADAO80%YIHOUZHONGFU1XIANGCAOZUOHUOZHEDONGCUN

比特棋牌(3)QUBUFENXIBAOXUANYEZHUANYIDAOXINDEPEIYANGMIN/PINGZHONG,TIANJIASHIDANGDEWANQUANPEIYANGJI,BAXIBAOXUANYEDAYUN,YUPEIYANGXIANGZHONGPEIYANG

比特棋牌(4)JINGXIAGUANCHAXIAOHUAQINGKUANG,ZAIHS505.TRENLINBAJIECHENGXIANWEIYANGXIBAOSHENGZHANGTEXINGBIANYUANSUOXIAO,TIEBISONGDONGSHI(BUJIANYIXIAOHUADAOXIBAOPIAOFU)QUDIAOYIMEI,JIA6-8mlWANQUANPEIYANGJI,QINGQINGCHUIDAXIBAOCENG,JINLIANGBAXIBAOCENGCHUILUO,CHUISAN。

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