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产品名称:

GIBCO胎牛血清

产品型号: 16250-078 产品时间: 2019-01-18
GIBCO16250-078超低IgG胎牛血清现货促销 细胞培养过程中,经常加入5%-20%的Gibco胎牛血清,较常使用的Gibco胎牛血清浓度是10%。高浓度的血清可能改变细胞的基因表达谱,影响后续实验的结果,我们在实验中使用10%的Gibco澳洲胎牛血清培养293T细胞,效果非常好。

产品概述

GIBCO16250-078超低IgG胎牛血清现货促销

Gibco牛血清污染噬菌体的问题。

1、问:有谁知道小牛血清制造过程中怎样能够避免噬菌体的污染,以及对已污染噬菌体的牛血清怎样能够除去噬菌体?

2、问:我也想知道,我用的血清中大部分都有噬菌体,它对细胞培养到底有什么影响?

答:不知道您究竟使用的什么血清,我们使用的Gibco胚胎干细胞专用血清16141079,没有任何噬菌体。建议您立刻更换胎牛血清,推荐您使用Gibco胎牛血清,具体的产品货号是:Gibco南美胎牛血清(Gibco南美血清10270106)、Gibco北美胎牛血清(Gibco北美血清16000044)、Gibco澳洲胎牛血清(Gibco澳洲血清10099141)、Gibco新西兰胎牛血清(Gibco新西兰血清10091148)、Gibco ES专用胎牛血清(Gibco ES专用血清16141079)。

GIBCO16250-078超低IgG胎牛血清现货促销

问:在1640里加血清时加多了,90ml里加了20ml Gibco新西兰血清10091148,约为18%,以前都是加10ml的,查了网上多建议用10%-15%,有关系吗?

答:我认为一般来说血清浓度的较大波动对细胞的状态影响较大,建议再加90ml1640调成10%。血清浓度大当然细胞状态感觉要好,但是高浓度的Gibco澳洲血清10099141可能改变细胞的基因表达谱,影响后续实验的结果。10%的浓度培养鼻烟癌细胞很好

 

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10270-106

比特棋牌 16000-044

10099-141

10091-148

16141-079

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YOUJI

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XUEYUAN

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≤50 EU/ml

比特棋牌≤5 EU/ml

≤10 EU/ml

≤10 EU/ml

≤10 EU/ml

XUEHONGSUSHUIPING

比特棋牌≤30 mg/dl

比特棋牌≤10 mg/dl

≤30 mg/dl

比特棋牌≤30 mg/dl

≤25 mg/dl

XUEQINGGUIGE

500ml

500ml

500ml

500ml

500ml

YUNSHUFANGSHI

GANBING

GANBING

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比特棋牌GbicoBEIMEITAINIUXUEQINGZHILIANGHENHAO,XUEYUANLAIYUANYUMEIGUO,GibcoTAINIUXUEQINGBEIMEINENGGOUMANZUGEZHONGPUTONGXIBAOHEYUANDAIXIBAO,GANXIBAO(PEITAIGANXIBAO,JIANCHONGZHIGANXIBAODENG)。TONGSHIYOUYUXUEHONGSUHENEIDUSUHANLIANGDOUFEICHANGDI,BIJIAOSHIHEYUPEIYANGBIJIAOMINGANHEJIAOGUIDEXIBAO。

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