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17504-044B-27® 添加剂是无血清添加剂

发布日期: 2019-04-09
浏览人气: 245

杭州仟诺生物科技有限公司17504-044B-27® 添加剂是无血清添加剂 N2等常用试剂大量现货备货,到货

 

B-27® 添加剂是无血清添加剂,用于海马神经元和其他中枢神经系统(CNS)神经元的生长和保持其短期或长期活性。B-27® 添加剂是50X 的液体,可以与Neurobasal® 培养基或Neurobasal®-A 培养基组合使用,用于神经细胞培养而不需要再添加饲养层细胞。

B-27® 添加剂适和应用于大多数神经细胞培养。对于特别的应用,我们提供 其他配方的B-27® 添加剂.

质量控制检测 
B-27® 添加剂 进行了18天妊娠的原代大鼠(Sprague Dawley) 的胚胎海马神经元生长检测。 通过了细菌和真菌的检测. B-27® 也做了1X 浓度的内毒素水平测试。 

仅供研究使用。除非另有说明,不得用于任何动物或人类治疗或诊断。

详细说明

B-27® 添加剂是无血清添加剂

常用规格

Cell Type:

Neuronal Cells

Product Size:

10 ml

Classification:

Serum-Free

Other Additives:

Vitamin A,

Antioxidant Cocktail,

Insulin

Purity or Quality Grade:

Cell Culture Grade

Green Features:

Sustainable packaging

Shipping Condition:

Dry Ice

Regulatory Statement:

For Research Use Only。 Not for use in diagnostic procedures。

引文和参考文献 (17)

  • D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels.

作者

Congar P; Bergevin A; Trudeau LE

杂志

J Neurophysiol (2002) 87:1046-1102

产品用途

Using a culture system in which isolated DAergic neurons establish self-innervating synapses ("autapses") that release both DA and glutamate, we studied the mechanism by which presynaptic D2 receptors inhibit glutamate-mediated excitatory postsynaptic currents (EPSCs).

ID:

PN64425

  • 比特棋牌Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice.

作者

Di Paolo Gilbert; Sankaranarayanan Sethuraman; Wenk Markus R; Daniell Laurie; Perucco Ezio; Caldarone Barbara J; Flavell Richard; Picciotto Marina R; Ryan Timothy A; Cremona Ottavio; De Camilli Pietro;

杂志

Neuron (2002) 33:789-804

产品用途

Primary cultures of cortical neurons were prepared. Cells were maintained up to 3 weeks in Neurobasal/B27 medium at 37°C in a 5% CO2 humidified incubator.

ID:

PN47418

  • Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21(CIP1/WAF1) in human neural stem cells.

作者

Kabos Peter; Kabosova Andrea; Neuman Toomas;

杂志

J Biol Chem (2002) 277:8763-8769

产品用途

Resected human fetal brain tissue was placed into ice cold DMEM/F-12 with penicillin/streptomycin for further dissection。 The tissue was cut into small pieces and trypsinized (0。02 mg/ml trypsin in versene (Invitrogen) at 37 °C for 10 min。 Cell suspension was centrifuged at 400 rpm for 5 min, the pellet was washed once with DMEM/F-12 and plated at a density of 5000-10,000 viable cells/ml in the growth medium DMEM/F-12, B27 supplement (Invitrogen)。 Cells were washed with PBS (Invitrogen)

ID:

PN63471

  • Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death.

作者

Lesuisse Christian; Martin Lee J;

杂志

J Neurobiol (2002) 51:9-23

产品用途

A long-term cell culture system was used to study maturation, aging, and death of cortical neurons。

ID:

PN60461

  • An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells.

作者

Joseph-Bravo Patricia; Pérez-Martínez Leonor; Lezama Leticia; Morales-Chapa Claudia; Charli Jean Louis;

杂志

Brain Res Brain Res Protoc (2002) 9:93-104

产品用途

Primary cultures of dispersed cells from fetal nervous tissue are extensively used for studying multiple neuronal properties

ID:

PN62028

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